Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis.
Identifieur interne : 004788 ( Main/Exploration ); précédent : 004787; suivant : 004789Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis.
Auteurs : H. Meyermans [Belgique] ; K. Morreel ; C. Lapierre ; B. Pollet ; A. De Bruyn ; R. Busson ; P. Herdewijn ; B. Devreese ; J. Van Beeumen ; J M Marita ; J. Ralph ; C. Chen ; B. Burggraeve ; M. Van Montagu ; E. Messens ; W. BoerjanSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 2000.
Descripteurs français
- KwdFr :
- Acide vanillique (analogues et dérivés), Acides caféiques (métabolisme), Acides coumariques (composition chimique), Acyl coenzyme A (métabolisme), Chromatographie en phase liquide à haute performance (MeSH), Conformation des glucides (MeSH), Glucosides (métabolisme), Lignine (métabolisme), Methyltransferases (métabolisme), Modèles chimiques (MeSH), Phénols (métabolisme), Protéines végétales (métabolisme), Régulation négative (MeSH), Spectrométrie de masse (MeSH), Spectroscopie par résonance magnétique (MeSH), Végétaux génétiquement modifiés (enzymologie).
- MESH :
- analogues et dérivés : Acide vanillique.
- composition chimique : Acides coumariques.
- enzymologie : Végétaux génétiquement modifiés.
- métabolisme : Acides caféiques, Acyl coenzyme A, Glucosides, Lignine, Methyltransferases, Phénols, Protéines végétales.
- Chromatographie en phase liquide à haute performance, Conformation des glucides, Modèles chimiques, Régulation négative, Spectrométrie de masse, Spectroscopie par résonance magnétique.
English descriptors
- KwdEn :
- Acyl Coenzyme A (metabolism), Caffeic Acids (metabolism), Carbohydrate Conformation (MeSH), Chromatography, High Pressure Liquid (MeSH), Coumaric Acids (chemistry), Down-Regulation (MeSH), Glucosides (metabolism), Lignin (metabolism), Magnetic Resonance Spectroscopy (MeSH), Mass Spectrometry (MeSH), Methyltransferases (metabolism), Models, Chemical (MeSH), Phenols (metabolism), Plant Proteins (metabolism), Plants, Genetically Modified (enzymology), Vanillic Acid (analogs & derivatives).
- MESH :
- chemical , analogs & derivatives : Vanillic Acid.
- chemical , chemistry : Coumaric Acids.
- chemical , metabolism : Acyl Coenzyme A, Caffeic Acids, Glucosides, Lignin, Methyltransferases, Phenols, Plant Proteins.
- enzymology : Plants, Genetically Modified.
- Carbohydrate Conformation, Chromatography, High Pressure Liquid, Down-Regulation, Magnetic Resonance Spectroscopy, Mass Spectrometry, Models, Chemical.
Abstract
Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) methylates, in vitro, caffeoyl-CoA and 5-hydroxyferuloyl-CoA, two possible precursors in monolignol biosynthesis in vivo. To clarify the in vivo role of CCoAOMT in lignin biosynthesis, transgenic poplars with 10% residual CCoAOMT protein levels in the stem xylem were generated. Upon analysis of the xylem, the affected transgenic lines had a 12% reduced Klason lignin content, an 11% increased syringyl/guaiacyl ratio in the noncondensed lignin fraction, and an increase in lignin-attached p-hydroxybenzoate but otherwise a lignin composition similar to that of wild type. Stem xylem of the CCoAOMT-down-regulated lines had a pink-red coloration, which coincided with an enhanced fluorescence of mature vessel cell walls. The reduced production of CCoAOMT caused an accumulation of O(3)-beta-d-glucopyranosyl-caffeic acid, O(4)-beta-d-glucopyranosyl-vanillic acid, and O(4)-beta-d-glucopyranosyl-sinapic acid (GSA), as authenticated by (1)H NMR. Feeding experiments showed that O(3)-beta-d-glucopyranosyl-caffeic acid and GSA are storage or detoxification products of caffeic and sinapic acid, respectively. The observation that down-regulation of CCoAOMT decreases lignin amount whereas GSA accumulates to 10% of soluble phenolics indicates that endogenously produced sinapic acid is not a major precursor in syringyl lignin biosynthesis. Our in vivo results support the recently obtained in vitro enzymatic data that suggest that the route from caffeic acid to sinapic acid is not used for lignin biosynthesis.
DOI: 10.1074/jbc.M006915200
PubMed: 10934215
Affiliations:
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Meyermans</name><affiliation wicri:level="4"><nlm:affiliation>Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, B-9000 Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, B-9000 Gent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Morreel, K" sort="Morreel, K" uniqKey="Morreel K" first="K" last="Morreel">K. Morreel</name></author><author><name sortKey="Lapierre, C" sort="Lapierre, C" uniqKey="Lapierre C" first="C" last="Lapierre">C. Lapierre</name></author><author><name sortKey="Pollet, B" sort="Pollet, B" uniqKey="Pollet B" first="B" last="Pollet">B. Pollet</name></author><author><name sortKey="De Bruyn, A" sort="De Bruyn, A" uniqKey="De Bruyn A" first="A" last="De Bruyn">A. De Bruyn</name></author><author><name sortKey="Busson, R" sort="Busson, R" uniqKey="Busson R" first="R" last="Busson">R. Busson</name></author><author><name sortKey="Herdewijn, P" sort="Herdewijn, P" uniqKey="Herdewijn P" first="P" last="Herdewijn">P. Herdewijn</name></author><author><name sortKey="Devreese, B" sort="Devreese, B" uniqKey="Devreese B" first="B" last="Devreese">B. Devreese</name></author><author><name sortKey="Van Beeumen, J" sort="Van Beeumen, J" uniqKey="Van Beeumen J" first="J" last="Van Beeumen">J. Van Beeumen</name></author><author><name sortKey="Marita, J M" sort="Marita, J M" uniqKey="Marita J" first="J M" last="Marita">J M Marita</name></author><author><name sortKey="Ralph, J" sort="Ralph, J" uniqKey="Ralph J" first="J" last="Ralph">J. Ralph</name></author><author><name sortKey="Chen, C" sort="Chen, C" uniqKey="Chen C" first="C" last="Chen">C. Chen</name></author><author><name sortKey="Burggraeve, B" sort="Burggraeve, B" uniqKey="Burggraeve B" first="B" last="Burggraeve">B. Burggraeve</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Messens, E" sort="Messens, E" uniqKey="Messens E" first="E" last="Messens">E. Messens</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="ISSN">0021-9258</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acyl Coenzyme A (metabolism)</term><term>Caffeic Acids (metabolism)</term><term>Carbohydrate Conformation (MeSH)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Coumaric Acids (chemistry)</term><term>Down-Regulation (MeSH)</term><term>Glucosides (metabolism)</term><term>Lignin (metabolism)</term><term>Magnetic Resonance Spectroscopy (MeSH)</term><term>Mass Spectrometry (MeSH)</term><term>Methyltransferases (metabolism)</term><term>Models, Chemical (MeSH)</term><term>Phenols (metabolism)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (enzymology)</term><term>Vanillic Acid (analogs & derivatives)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide vanillique (analogues et dérivés)</term><term>Acides caféiques (métabolisme)</term><term>Acides coumariques (composition chimique)</term><term>Acyl coenzyme A (métabolisme)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Conformation des glucides (MeSH)</term><term>Glucosides (métabolisme)</term><term>Lignine (métabolisme)</term><term>Methyltransferases (métabolisme)</term><term>Modèles chimiques (MeSH)</term><term>Phénols (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Régulation négative (MeSH)</term><term>Spectrométrie de masse (MeSH)</term><term>Spectroscopie par résonance magnétique (MeSH)</term><term>Végétaux génétiquement modifiés (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Vanillic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Coumaric Acids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyl Coenzyme A</term><term>Caffeic Acids</term><term>Glucosides</term><term>Lignin</term><term>Methyltransferases</term><term>Phenols</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide vanillique</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acides coumariques</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides caféiques</term><term>Acyl coenzyme A</term><term>Glucosides</term><term>Lignine</term><term>Methyltransferases</term><term>Phénols</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Conformation</term><term>Chromatography, High Pressure Liquid</term><term>Down-Regulation</term><term>Magnetic Resonance Spectroscopy</term><term>Mass Spectrometry</term><term>Models, Chemical</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Conformation des glucides</term><term>Modèles chimiques</term><term>Régulation négative</term><term>Spectrométrie de masse</term><term>Spectroscopie par résonance magnétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) methylates, in vitro, caffeoyl-CoA and 5-hydroxyferuloyl-CoA, two possible precursors in monolignol biosynthesis in vivo. To clarify the in vivo role of CCoAOMT in lignin biosynthesis, transgenic poplars with 10% residual CCoAOMT protein levels in the stem xylem were generated. Upon analysis of the xylem, the affected transgenic lines had a 12% reduced Klason lignin content, an 11% increased syringyl/guaiacyl ratio in the noncondensed lignin fraction, and an increase in lignin-attached p-hydroxybenzoate but otherwise a lignin composition similar to that of wild type. Stem xylem of the CCoAOMT-down-regulated lines had a pink-red coloration, which coincided with an enhanced fluorescence of mature vessel cell walls. The reduced production of CCoAOMT caused an accumulation of O(3)-beta-d-glucopyranosyl-caffeic acid, O(4)-beta-d-glucopyranosyl-vanillic acid, and O(4)-beta-d-glucopyranosyl-sinapic acid (GSA), as authenticated by (1)H NMR. Feeding experiments showed that O(3)-beta-d-glucopyranosyl-caffeic acid and GSA are storage or detoxification products of caffeic and sinapic acid, respectively. The observation that down-regulation of CCoAOMT decreases lignin amount whereas GSA accumulates to 10% of soluble phenolics indicates that endogenously produced sinapic acid is not a major precursor in syringyl lignin biosynthesis. Our in vivo results support the recently obtained in vitro enzymatic data that suggest that the route from caffeic acid to sinapic acid is not used for lignin biosynthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10934215</PMID><DateCompleted><Year>2001</Year><Month>01</Month><Day>08</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9258</ISSN><JournalIssue CitedMedium="Print"><Volume>275</Volume><Issue>47</Issue><PubDate><Year>2000</Year><Month>Nov</Month><Day>24</Day></PubDate></JournalIssue><Title>The Journal of biological chemistry</Title><ISOAbbreviation>J Biol Chem</ISOAbbreviation></Journal><ArticleTitle>Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis.</ArticleTitle><Pagination><MedlinePgn>36899-909</MedlinePgn></Pagination><Abstract><AbstractText>Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) methylates, in vitro, caffeoyl-CoA and 5-hydroxyferuloyl-CoA, two possible precursors in monolignol biosynthesis in vivo. To clarify the in vivo role of CCoAOMT in lignin biosynthesis, transgenic poplars with 10% residual CCoAOMT protein levels in the stem xylem were generated. Upon analysis of the xylem, the affected transgenic lines had a 12% reduced Klason lignin content, an 11% increased syringyl/guaiacyl ratio in the noncondensed lignin fraction, and an increase in lignin-attached p-hydroxybenzoate but otherwise a lignin composition similar to that of wild type. Stem xylem of the CCoAOMT-down-regulated lines had a pink-red coloration, which coincided with an enhanced fluorescence of mature vessel cell walls. The reduced production of CCoAOMT caused an accumulation of O(3)-beta-d-glucopyranosyl-caffeic acid, O(4)-beta-d-glucopyranosyl-vanillic acid, and O(4)-beta-d-glucopyranosyl-sinapic acid (GSA), as authenticated by (1)H NMR. Feeding experiments showed that O(3)-beta-d-glucopyranosyl-caffeic acid and GSA are storage or detoxification products of caffeic and sinapic acid, respectively. The observation that down-regulation of CCoAOMT decreases lignin amount whereas GSA accumulates to 10% of soluble phenolics indicates that endogenously produced sinapic acid is not a major precursor in syringyl lignin biosynthesis. Our in vivo results support the recently obtained in vitro enzymatic data that suggest that the route from caffeic acid to sinapic acid is not used for lignin biosynthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meyermans</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, B-9000 Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morreel</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Lapierre</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Pollet</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>De Bruyn</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Busson</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Herdewijn</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Devreese</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Van Beeumen</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Marita</LastName><ForeName>J M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Burggraeve</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Van Montagu</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Messens</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>W</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Biol Chem</MedlineTA><NlmUniqueID>2985121R</NlmUniqueID><ISSNLinking>0021-9258</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C081711">4-O-beta-glucopyranosyl caffeic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000214">Acyl Coenzyme A</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002109">Caffeic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003373">Coumaric Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005960">Glucosides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>53034-79-0</RegistryNumber><NameOfSubstance UI="C058645">caffeoyl-coenzyme A</NameOfSubstance></Chemical><Chemical><RegistryNumber>59862-14-5</RegistryNumber><NameOfSubstance UI="C090959">5-hydroxyferuloyl-coenzyme A</NameOfSubstance></Chemical><Chemical><RegistryNumber>68A28V6010</RegistryNumber><NameOfSubstance UI="C073734">sinapinic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D008780">Methyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.104</RegistryNumber><NameOfSubstance UI="C059825">caffeoyl-CoA O-methyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GM8Q3JM2Y8</RegistryNumber><NameOfSubstance UI="D014641">Vanillic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000214" MajorTopicYN="N">Acyl Coenzyme A</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002109" MajorTopicYN="N">Caffeic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002236" MajorTopicYN="N">Carbohydrate Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003373" MajorTopicYN="N">Coumaric Acids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="Y">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005960" MajorTopicYN="N">Glucosides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009682" MajorTopicYN="N">Magnetic Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008780" MajorTopicYN="N">Methyltransferases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008956" MajorTopicYN="N">Models, Chemical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014641" MajorTopicYN="N">Vanillic Acid</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>8</Month><Day>10</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>8</Month><Day>10</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10934215</ArticleId><ArticleId IdType="doi">10.1074/jbc.M006915200</ArticleId><ArticleId IdType="pii">M006915200</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name><name sortKey="Burggraeve, B" sort="Burggraeve, B" uniqKey="Burggraeve B" first="B" last="Burggraeve">B. Burggraeve</name><name sortKey="Busson, R" sort="Busson, R" uniqKey="Busson R" first="R" last="Busson">R. Busson</name><name sortKey="Chen, C" sort="Chen, C" uniqKey="Chen C" first="C" last="Chen">C. Chen</name><name sortKey="De Bruyn, A" sort="De Bruyn, A" uniqKey="De Bruyn A" first="A" last="De Bruyn">A. De Bruyn</name><name sortKey="Devreese, B" sort="Devreese, B" uniqKey="Devreese B" first="B" last="Devreese">B. Devreese</name><name sortKey="Herdewijn, P" sort="Herdewijn, P" uniqKey="Herdewijn P" first="P" last="Herdewijn">P. Herdewijn</name><name sortKey="Lapierre, C" sort="Lapierre, C" uniqKey="Lapierre C" first="C" last="Lapierre">C. Lapierre</name><name sortKey="Marita, J M" sort="Marita, J M" uniqKey="Marita J" first="J M" last="Marita">J M Marita</name><name sortKey="Messens, E" sort="Messens, E" uniqKey="Messens E" first="E" last="Messens">E. Messens</name><name sortKey="Morreel, K" sort="Morreel, K" uniqKey="Morreel K" first="K" last="Morreel">K. Morreel</name><name sortKey="Pollet, B" sort="Pollet, B" uniqKey="Pollet B" first="B" last="Pollet">B. Pollet</name><name sortKey="Ralph, J" sort="Ralph, J" uniqKey="Ralph J" first="J" last="Ralph">J. Ralph</name><name sortKey="Van Beeumen, J" sort="Van Beeumen, J" uniqKey="Van Beeumen J" first="J" last="Van Beeumen">J. Van Beeumen</name><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Meyermans, H" sort="Meyermans, H" uniqKey="Meyermans H" first="H" last="Meyermans">H. Meyermans</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis.
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